Publications

Featured publications

Zytnick AM, Good NM, Barber CC, Tri M-P, Gutenthaler SM, Zhang W, Daumann LJ, Martinez-Gomez NC. (2022) Identification of a biosynthetic gene cluster encoding a novel lanthanide chelator in Methylorubrum extorquens AM1. bioRxiv. https://doi.org/10.1101/2022.01.19.476857

Good NM, Lee H, Hawker ER, Su MZ, Gilad AA, Martinez-Gomez NC. (2022) Hyperaccumulation of Gadolinium by Methylorubrum extorquens AM1 Reveals Impacts of Lanthanides on Cellular Processes Beyond Methylotrophy. Front Microbiol 13:820327. https://doi.org/10.3389/fmicb.2022.820327

Good NM, Fellner M, Demirer K, Hu J, Hausinger RP, Martinez-Gomez NC. (2020) Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function. J Biol Chem 295:8272-8284. https://doi.org/10.1074/jbc.RA120.013227

Roszczenko-Jasińska P, Vu H, Subuyuj GA, Crisostomo RV, Cai J, Raghuraman C, Ayala EM, Clippartd EJ, Lien NF, Ngo RT, Yarza F, Hoeber CA, Martinez-Gomez NC, Skovran E. (2020) Gene products and processes contributing to lanthanide homeostasis and methanol metabolism in Methylorubrum extorquens AM1. Scientific Reports 10:12633. https://doi.org/10.1038/s41598-020-69401-4

Good NM, Vu HN, Suriano CJ, Subuyuj GA, Skovran E, Martinez-Gomez NC. (2016) Pyrroloquinoline Quinone Ethanol Dehydrogenase in Methylobacterium extorquens AM1 Extends Lanthanide-Dependent Metabolism to Multicarbon Substrates. J Bacteriol 198(22):3109-3118. https://doi.org/10.1128/JB.00478-16

Skovran E, Martinez-Gomez NC. (2015) Microbiology. Just add lanthanides. Science 348(6237):862-3. https://doi.org/10.1126/science.aaa9091

All publications

Lee HD, Grady CJ, Krell K, Strebeck C, Good NM, Martinez-Gomez NC, Gilad AA. (2023) A Novel Protein for the Bioremediation of Gadolinium Waste. bioRxiv [Preprint]. https://doi.org/10.1101/2023.01.05.522788.

Leducq JB, Sneddon D, Santos M, Condrain-Morel D, Bourret G, Martinez-Gomez NC, Lee JA, Foster JA, Stolyar S, Shapiro BJ, Kembel SW, Sullivan JM, Marx CJ. (2022) Comprehensive Phylogenomics of Methylobacterium Reveals Four Evolutionary Distinct Groups and Underappreciated Phyllosphere Diversity. Genome Biol Evol 14(8). https://doi.org/10.1093/gbe/evac123.

Jones PA, Frischer D, Mueller S, Le T, Schwanes A, Govindaraju A, Shalvarjian K, Leducq JB, Marx CJ, Martinez-Gomez NC, Lee JA. (2022) Methylothon: a Versatile Course-Based High School Research Experience in Microbiology and Bioinformatics with Pink Bacteria. J Microbiol Biol Educ 23(2). https://doi.org/10.1128/jmbe.00227-21

Govindaraju A, Good NM, Zytnick AM, Martinez-Gomez NC. (2022) Employing methylotrophs for a green economy: one-carbon to fuel them all and through metabolism redesign them. Curr Opin Microbiol. 67:102145. https://doi.org/10.1016/j.mib.2022.102145.

Daumann LJ, Pol A, Op den Camp HJM, Martinez-Gomez NC. (2022) A perspective on the role of lanthanides in biology: Discovery, open questions and possible applications. Adv Microb Physiol 81:1-24. https://doi.org/10.1016/bs.ampbs.2022.06.001

Good NM, Martinez-Gomez NC. (2021) Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1. Methods Enzymol 650:97-118. https://doi.org/10.1016/bs.mie.2021.02.001

WORK at MSU 2015-2020

Roszczenko-Jasińska P, Vu H, Subuyuj GA, Crisostomo RV, Cai J, Raghuraman C, Ayala EM, Clippartd EJ, Lien NF, Ngo RT, Yarza F, Hoeber CA, Martinez-Gomez NC, Skovran E. (2020) Lanthanide transport, storage, and beyond: genes and processes contributing to XoxF function in Methylorubrum extorquens AM1. Scientific Reports 10:12633. https://doi.org/10.1038/s41598-020-69401-4

Jahn B, Jonasson NSW, Hu H, Pol A, Good NM, Op den Camp HJM, Martinez-Gomez NC, Daumann L. (2020) Understanding the Chemistry of Artificial Electron Acceptors and Redox Dyes – A Study with Lanthanide-Dependent Methanol Dehydrogenases. J Biol Inorg Chem 25:199-212. https://doi.org/10.1007/s00775-020-01752-9

Skovran E, Raghuraman C, Martinez-Gomez NC. (2019) Lanthanides in Methylotrophy. Curr Issues Mol Biol 33:101–116. https://doi.org/10.21775/cimb.033.101

Good NM, Moore RS, Suriano CJ, Martinez-Gomez NC. (2019) Contrasting in vitro and in vivo methanol oxidation activities of lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium extorquens AM1. Scientific Reports 9(1):4248. https://doi.org/10.1038/s41598-019-41043-1

Hogendoorn C, Roszczenko-Jasińska P, Martinez-Gomez NC, de Graaff J, Grassl P, Pol A, Op den Camp HJM, Daumann LJ. (2018) Facile Arsenazo III-Based Assay for Monitoring Rare Earth Element Depletion from Cultivation Media for Methanotrophic and Methylotrophic Bacteria. Appl Environ Microbiol 84(8):e02887-17. https://doi.org/10.1128/AEM.02887-17

Martinez-Gomez NC, Vu HN, Skovran E. (2016) Lanthanide Chemistry: From Coordination in Chemical Complexes Shaping Our Technology to Coordination in Enzymes Shaping Bacterial Metabolism. Inorg Chem 55(20):10083-10089. https://doi.org/10.1021/acs.inorgchem.6b00919

Agashe D, Sane M, Phalnikar K., Diwan GD, Habibullah A, Martinez-Gomez NC, Sahasrabuddhe V, Polachek W, Wang J, Chubiz LM, Marx CJ. (2016) Large-Effect Beneficial Synonymous Mutations Mediate Rapid and Parallel Adaptation in a Bacterium. Mol Biol Evol 33(6):1542-53. https://doi.org/10.1093/molbev/msw035

Vu HN, Subujuj GA, Vijayakumar S, Good NM, Martinez-Gomez NC, Skovran E. (2016) Lanthanide-Dependent Regulation of Methanol Oxidation Systems in Methylobacterium extorquens AM1 and Their Contribution to Methanol Growth. J Bacteriol 198(8):1250-9. https://doi.org/10.1128/JB.00937-15

Skovran E, Martinez-Gomez NC. (2015) Microbiology. Just add lanthanides. Science 348(6237):862-3. https://doi.org/10.1126/science.aaa9091

Postdoctorate work 2010-2015

Martinez-Gomez NC, Good NM, Lidstrom ME. (2015) Methenyl-dephosphotetrahydromethanopterin is a regulatory signal for acclimation to changes in substrate availability in Methylobacterium extorquens AM1. J Bacteriol 197(12):2020-6. https://doi.org/10.1128/JB.02595-14

Good NM, Lamb A, Beck DAC, Martinez-Gomez NC, Kalyuzhnaya MG. (2015) C1-Pathways in Methyloversatilis universalis FAM5: Genome Wide Gene Expression and Mutagenesis Studies. Microorganisms 3(2):175-97. https://doi.org/10.3390/microorganisms3020175

Good NM, Martinez-Gomez NC, Beck DA, Lidstrom ME. (2015) Ethylmalonyl coenzyme A mutase operates as a metabolic control point in Methylobacterium extorquens AM1. J Bacteriol 197(4):727-35. https://doi.org/10.1128/JB.02478-14

Martinez-Gomez NC, Nguyen S, Lidstrom ME. (2013) Elucidation of the role of the methylene-tetrahydromethanopterin dehydrogenase MtdA in the tetrahydromethanopterin- dependent oxidation pathway in Methylobacterium extorquens AM1. J Bacteriol 195(10):2359-67. https://doi.org/10.1128/JB.00029-13

Agashe D, Martinez-Gomez NC, Drummond DA, Marx CJ. (2013) Good codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzyme. Mol Biol Evol 30(3):549-60. https://doi.org/10.1093/molbev/mss273

Graduate work 2004-2009

Martinez-Gomez NC, Palmer LD, Vivas E, Roach PL, Downs DM. (2011) The rhodanese domain of Thil is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica. J Bacteriol 193(18):4582-7. https://doi.org/10.1128/JB.05325-11

Martinez-Gomez NC, Poyner RR, Mansoorabadi SO, Reed GH, Downs DM. (2009) Reaction of AdoMet with ThiC generates a backbone free radical. Biochemistry 48(2):217-9. https://doi.org/10.1021/bi802154j

Martinez-Gomez NC, Downs DM. (2008) ThiC is an [Fe-S] cluster protein that requires AdoMet to generate the 4-amino-5 hydroxymethyl-2-methylpyrimidine moiety in thiamin synthesis. Biochemistry 47(35):9054-6. https://doi.org/10.1021/bi8010253

Martinez-Gomez NC, Downs DM. (2004) Mutational analysis of ThiH, a member of the radical S-adenosylmethionine (AdoMet) protein superfamily. J Biol Chem 279(39):40505-10. https://doi.org/10.1074/jbc.M403985200